Study of regulation of physiological processes at the cellular, tissue and organism level by posttranslational protein arginylation
Research focus of my laboratory is to understand the role of posttranslational protein arginylation, an emerging PTM, in regulation of physiological processes at the cellular, tissue
and organism level.
One of the directions of our research is to understand the role of arginylation in cellular stress responses. Stress response is a very important physiological process which protects cells
from environmental or physiological insults. We are using heat stress and oxidative stress as a model system to investigate the role of protein arginylation in regulation of stress responses. Our study in this direction has identified an arginylation dependent novel pathway in regulation of inducible Hsp70 mRNA stability. Our study showed that, RNA binding activity and stability of key RNA metabolizing protein HuR is regulated by protein arginylation which in turn regulates alternate polyadenylation and stability of Hsp70 transcripts. Studies in this direction in my laboratory are aimed at identifying novel pathways of stress regulation through arginylation and its role in human diseases and aging.
Deka K, Saha S. Heat stress induced arginylation of HuR promotes alternative polyadenylation of Hsp70. 3 by regulating HuR stability and RNA binding. Cell Death & Differentiation. 2021 Feb;28(2):730-47. IF: 15.83
Deka K,Saha S.Arginylation: a new regulator of mRNA stability and heat stress response. Cell Death & Disease. 2017 Feb;8(2):e2604. IF: 8.4
Deka K, Singh A, Chakraborty S, Mukhopadhyay R, Saha S. Protein arginylation regulates cellular stress response by stabilizing HSP70 and HSP40 transcripts. Cell Death Discovery. 2016 Oct 3;2(1):1-8. IF: 5.2
In another direction of study in my laboratory we are testing the hypothesis that, protein arginylation has a key role in cellular differentiation of diverse lineages. In this direction we have
shown that arginylation promotes adipogenesis by regulating expression of key adipogenic factor PPARγ at the transcript and protein level. In an ongoing study we have also observed that, this modification is crucial for osteogenesis as well. With the goal of utilizing our findings to develop novel therapeutic approaches for treatment of obesity, we are also involved in identifying novel phyto-compounds to regulate arginyltransferase (ATE1) activity and adipocyte differentiation.
Singh A, Borah AK, Deka K, Gogoi AP, Verma K, Barah P, Saha S. Arginylation regulates adipogenesis by regulating expression of PPARγ at transcript and protein level. Biochimica et Biophysica Acta (BBA)-Molecular and Cell Biology of Lipids. 2019 Apr 1;1864(4):596-607. IF: 4.69
Borah AK, Singh A, Yasmin R, Doley R, Mattaparthi VS, Saha S. 1α, 25-dihydroxy Vitamin D3 containing fractions of Catharanthus roseus leaf aqueous extract inhibit preadipocyte differentiation and induce lipolysis in 3T3-L1 cells. BMC complementary and alternative medicine. 2019 Dec;19(1):1-7. IF: 3.65
Saha S, Wang J, Buckley B, Wang Q, Lilly B, Chernov M, Kashina A. Small molecule inhibitors of arginyltransferase regulate arginylation-dependent protein degradation, cell motility, and angiogenesis. Biochemical pharmacology. 2012 Apr 1;83(7):866-73. IF: 5.85
Saha S, Wong CC, Xu T, Namgoong S, Zebroski H, Yates III JR, Kashina A. Arginylation and methylation double up to regulate nuclear proteins and nuclear architecture in vivo. Chemistry & biology. 2011 Nov 23;18(11):1369-78. IF: 8.11
Zhang F, Saha S, Shabalina SA, Kashina A. Differential arginylation of actin isoforms is regulated by coding sequence–dependent degradation. Science. 2010 Sep 17;329(5998):1534-7. IF: 47.7
Saha S, Mundia MM, Zhang F, Demers RW, Korobova F, Svitkina T, Perieteanu AA, Dawson JF, Kashina A. Arginylation regulates intracellular actin polymer level by modulating actin properties and binding of capping and severing proteins. Molecular biology of the cell. 2010 Apr 15;21(8):1350-61. IF: 3.53
Projects
Sl. No.
Title
Duration
Role as PI/Co-PI
Agency
1.
Study on the regulation of adipose tissue development and function by post-translational protein arginylation.
2013-2016
PI
DBT
2.
Role of protein arginylation in cellular stress responses….. in arginylation deficient cell culture system
2017-2020
PI
SERB
3.
Study on the role of protein arginylation in regulation of adipo-osteogenic differentiation
2017-2020
PI
SERB
4.
"Role of Protein arginylation in the regulation………cells"
2022-2025
PI
SERB
Teachings
BTC01 Life Science
BTC401 Molecular Biology and rDNA Tech
BTC502 Cell and tissue culture
BTE614 Molecular Virology
BTE718 Proteomics and protein engineering
BT1103 Cell and Molecular Biology
BT2104 Genetic Engineering
UG Students
Data Not Found!
PG Students
Data Not Found!
Doctoral Students
Rahul Naga
JRF
Protein arginylation
Rahul Naga
JRF
Protein arginylation
Projects
Sl.No.
Title
Name of the PI
Name of the CoPIs
Funding Agency
Amount (Rs.)
Project Type
Project Status
Date of Initiation
Date of Completion
1
Role of protein arginylation in the regulation of IL-lβ expression and inflammatory response in macrophages and airway epithelial cells.
Saha S.
NA
SERB
5,500,000
Sponsored
Ongoing
2022
2
Screening of phytochemicals against metabolic, chronic inflammation and degenerative disorders
Saha, S., Mukherjee, O., De, D.
NA
RIG (NITDGP)
1,500,000
Sponsored
Completed
2019
2022
3
Role of protein arginylation in cellular stress responses: A study using heat stress and oxidative stress models in arginylation deficient cell culture system.
Saha S.
NA
SERB, DST. Gov. of India
4,876,000
Sponsored
Completed
2017
2020
Projects
Sl.No.
Title
Name of the PI
Name of the CoPIs
Funding Agency
Amount (Rs.)
Project Type
Project Status
Date of Initiation
Date of Completion
1
Role of protein arginylation in the regulation of IL-lβ expression and inflammatory response in macrophages and airway epithelial cells.
Saha S.
NA
SERB
5,500,000
Sponsored
Ongoing
2022
2
Screening of phytochemicals against metabolic, chronic inflammation and degenerative disorders
Saha, S., Mukherjee, O., De, D.
NA
RIG (NITDGP)
1,500,000
Sponsored
Completed
2019
2022
3
Role of protein arginylation in cellular stress responses: A study using heat stress and oxidative stress models in arginylation deficient cell culture system.
ERP